Figure 2 | Scientific Reports

Figure 2

From: The mechanical microenvironment regulates ovarian cancer cell morphology, migration, and spheroid disaggregation

Figure 2

Cell size and focal adhesion morphology positively correlate with substrate rigidity. (A) SKOV-3 cells plated on FN-coated glass (top), 25 kPa, and 3 kPa hydrogels (middle and bottom) were fixed and stained with phalloidin and paxillin (Pxn) antibody to visualize F-actin stress fibers and focal adhesion complexes (FA) respectively. (B–E) Immunofluorescence images were analyzed for cells on each substrate to determine cell size (B), number of FA/cells (C), FA size (D), and FA aspect ratio (E). Graphs show all measured values as colored symbols as well as mean values ± s.d. as black bars (n = 50 cells (B and C) and 750 FA each for glass and 25 kPa and 15 FA for 3 kPa (C and D); *p < 0.0001 and #p = 0.0261, using the Mann-Whitney test).

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