Figure 5
Substrate rigidity regulates ovarian cancer cell migration. (A) SKOV-3 cells plated on FN-coated 3, 25, and 125 kPa hydrogels were monitored by live-cell microscopy (capture rate = 4 frames/h) and nuclei were tracked using AIVIA software. Consecutive frames of time-lapse movies of were color-coded (as depicted by the “0 h–14 h” scale bar) and maximum intensity projections of the stacks were generated to show the cumulative cell tracks over 14 h. (B) Maximum displacements were calculated by determining the largest Euclidean distance between the position of each cell at t = 0 and its position at each frame of the time lapse image. The graph depicts all of the data points (colored symbols), with box & whisker plots showing maximum & minimum values (whiskers), the 25th to 75th percentiles (box), and the median value (box line). (n = 40 cells from 3 experiments; *p < 0.0001; **p = 0.002 using a Mann-Whitney test). (C) The percent of migrating cells was calculated by determining the number of cells with a maximum displacement >50 µm versus the total number of cells per movie. (n = 79, 159, and 180 cells from 3 experiments for 3, 25, and 125 kPa samples, respectively; *p = 0.008).
