Figure 7
Disaggregation of ovarian cancer spheroids is dependent on substrate rigidity. (A) SKOV3 multicellular spheroids were plated on FN-coated hydrogels with rigidities of 3 kPa, 25 kPa, and 125 kPa and imaged at 2 h and 20 h post plating. Red lines in the 20 h panels help visualize the boundaries of the tumor spheroids. (B) The graph depicts the change in area of each spheroid measured at 2 h and 20 h by manually outlining spheroids at the indicated time points and calculating the area using ImageJ (n = 24 spheroids for each condition; *p < 0.0001; n.s. = not significant).
