Figure 1

HuaiQihuang extractum (HQH) or Dexamethasone (Dex) pretreatment attenuated acute renal injury induced by cisplatin (Cis). (A) Scheme: The group of HQH + Cis received 6 g/kg HQH for two consecutive days before intraperitoneal injection with 20 mg/kg cisplatin and the group of Dex + Cis intraperitoneally injected with 4 mg/kg Dex 30 minutes before intraperitoneal injection with cisplatin. (B) Serum urea was measured at day 3 after cisplatin injection (*P < 0.05, **P < 0.01, n = 5/group). (C) Representative medullary congestion after Cisplatin injury with or without HQH and Dex pretreatment. Graphs indicate congestion area. (D) Representative photomicrographs and graphs of Cisplatin induced kidney injury, with or without HQH and Dex pretreatment. Injured tubules was showed by PAS staining, Bar = 50 μm. LTL (lotus tetragonolobus lectin) identifying proximal tubules (green), Bar = 100 μm. Kim-1 expression (red) identifying tubular injury, Bar = 50 μm. Statistics analyzed using the Mann-Whitney U test. **P < 0.01, ****P < 0.0001, n = 5/group. Values are means ± SEM.