Figure 2

HQH pretreatment protected mice from cisplatin induced tubular apoptosis and necroptosis, but not improved renal tubular epithelial cell proliferation. (A) Representative photomicrographs and graphs of immunofluorescence staining for Ki-67. Bar = 50 μm. (**P < 0.01 versus control, n = 8/group). Ki-67 was stained in red and nucleus was counterstained in blue by DAPI. (B) Representative photomicrographs and graphs of TUNEL (green) assay for analysis of tubular apoptosis (**P < 0.01, n = 5/group). Bar = 50 μm. (C) Representative Western blot and graphs for pro-apoptotic proteins Bax (C and D, *P < 0.05, n = 3/group), anti-apoptotic protein Bcl-2 (E and F, *P < 0.05, n = 3/group) and the key element of necroptosis RIP3 (*P < 0.05, n = 3/group) and MLKL (*P < 0.05, n = 3/group). Each group of blots is the same exposure of a gel, each group of results repeated more than three times.