Figure 6

HQH and Dex pretreatment attenuate renal injuries induced by folic acid (FA) or unilateral kidney ischemia reperfusion (IRI). (A) Scheme: Male C57BL/6 mice with body weight about 25 g underwent intraperitoneal injection with NaHCO3(150 mM) or 150 mg/kg folic acid (n = 4/group). Mice received gavage of 6 g/kg HQH extractum every day or one dose of Dex with 4 mg/kg before intraperitoneal injection with 150 mg/kg folic acid. (B) Representative photomicrographs and graphs of kidney injury outcomes induced by folic acid (*P < 0.05, n = 4/group). Bar = 100 μm. Serum urea was measured at day 3 (*P < 0.05, n = 4/group). (C) Scheme: Male C57BL/6 mice underwent left kidney Ischemia Reperfusion Injury (IRI) (ischemia 20 min, n = 5/group). The group of HQH + IRI mice received 10 g/kg HQH extractum gavage two consecutive days before IRI surgery on left kidney and sacrificed three days later (ischemia 20 min, n = 5/group). The group of Dex + IRI mice intraperitoneal injected with 4 mg/kg Dex one hour before left kidney IRI surgery (ischemia 20 min, n = 5/group). (D) Representative photomicrographs and graphs of the kidney outcomes of the IRI models (*P < 0.05,**P < 0.01, n = 4 ~ 5/group). Bar = 50 μm. Serum urea was measured at day 3 after IRI (*P < 0.05, n = 4 ~ 5/group).