Figure 1 | Scientific Reports

Figure 1

From: Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages

Figure 1

Protein and sterol quantification in EV from H. capsulatum. Yeast cells were incubated with or without 6 and 20 μg/mL of 6B7 and 7B6 mAb. The EV protein content was determined by BCA assay (A). EV sterol quantification was performed using Amplex reagent kit (B). Graphs represent means and standard deviation from at least two independent EV isolations and all the analyses were performed in duplicate. *p ≤ 0.05, compared to the untreated control group. #p ≤ 0.05, compared to the groups 6B7 and 7B6 at 6 µg/mL. ϕp ≤0.05, compared to 6B7 mAb treatment at 20 µg/mL.

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