Figure 6
Reactive oxygen species generation by EV-treated macrophages. BMDMs were loaded with H2DCFDA and then treated or not with EV from H. capsulatum opsonized or not with 6 or 20 μg/mL of 6B7 or 7B6 mAb, prior to the incubation with H. capsulatum yeast cells. ROS generation was measured in a microplate reader 2 hours after the incubation of the yeast cells. Graphs show the mean and standard errors from 2 independent experiments performed in quadruples. ****p < 0.0001 compared to macrophages without EV treatment. +p < 0.05; ++p < 0.01 and ++++p < 0.0001 compared to macrophages treated with control EV, by One-way ANOVA followed by Tukey’s multiple comparisons test.
