Table 1 KEGG pathway enrichment analysis of proteins with altered abundance in the extracellular vesicles after treatment of H. capsulatum. The table shows the number of proteins in each treatment on significantly-enriched pathways (p ≤ 0.05 by Fisher’s exact test and 2 folds enriched compared to the genome background). The analysis only considered proteins with significantly-altered abundance after treatment of H. capsulatum with 6B7 and 7B6 mAbs in the concentration of 6 and 20 µg/mL.

From: Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages

Pathway

Number of Significant proteins

6B7/Control

7B6/Control

6 µg/uL

20 µg/uL

6 µg/uL

20 µg/uL

Down

Up

Down

Up

Down

Up

Down

Up

80

30

5

22

24

73

41

55

Fold enrichment

Fisher test

2-Oxocarboxylic acid metabolism

4.5

9.9E-03

2

    

2

  

Alanine, aspartate and glutamate metabolism

3.8

3.6E-02

1

1

   

1

  

Amino sugar and nucleotide sugar metabolism

4.0

1.4E-02

1

  

1

2

 

2

1

Arginine and proline metabolism

4.8

2.0E-02

1

   

1

1

1

1

Arginine biosynthesis

6.1

1.1E-02

1

1

   

1

  

Biosynthesis of amino acids

7.0

4.8E-12

10

2

 

3

2

5

2

4

Biosynthesis of antibiotics

4.8

8.4E-12

13

3

 

3

5

7

4

4

Biosynthesis of secondary metabolites

4.0

2.1E-11

14

3

 

4

5

9

5

5

Carbon metabolism

6.1

5.2E-09

9

1

 

3

3

4

3

3

Citrate cycle (TCA cycle)

5.5

5.0E-03

4

       

Cysteine and methionine metabolism

7.6

7.1E-06

5

      

3

Fatty acid degradation

10.8

6.3E-05

2

   

1

2

2

2

Fructose and mannose metabolism

5.8

1.2E-02

3

  

1

2

   

Glutathione metabolism

4.8

2.0E-02

2

      

1

Glycolysis / Gluconeogenesis

11.5

6.5E-09

8

  

2

4

1

3

2

Glyoxylate and dicarboxylate metabolism

8.2

6.3E-05

4

    

1

1

 

Lysine biosynthesis

10.1

2.6E-03

2

1

      

Lysine degradation

7.0

2.0E-03

2

1

  

1

 

2

 

MAPK signaling pathway - yeast

2.6

4.7E-02

3

   

1

1

2

 

Metabolic pathways

3.2

6.8E-18

25

9

 

6

9

18

10

12

Methane metabolism

8.8

1.9E-04

3

   

1

2

1

1

mRNA surveillance pathway

5.1

8.5E-04

2

1

 

1

 

2

 

1

Nitrogen metabolism

7.4

6.5E-03

1

1

     

1

Oxidative phosphorylation

3.7

5.2E-04

3

3

  

1

3

2

 

Pentose and glucuronate interconversions

6.1

3.7E-02

2

   

1

1

1

 

Pentose phosphate pathway

6.7

2.4E-03

2

1

 

1

1

1

 

1

Phagosome

7.4

3.2E-05

3

1

   

2

 

2

Proteasome

3.4

4.8E-02

1

1

 

1

 

1

  

Protein processing in endoplasmic reticulum

3.4

3.2E-03

7

   

2

 

3

 

Pyruvate metabolism

6.6

7.5E-04

4

   

1

 

2

 

Riboflavin metabolism

5.7

4.2E-02

   

1

1

 

1

1

Ribosome

4.1

5.3E-05

5

1

   

1

4

2

Spliceosome

3.0

6.8E-03

2

2

 

1

   

2

Tryptophan metabolism

4.4

2.5E-02

2

   

1

 

2

 

Tyrosine metabolism

7.4

1.2E-04

3

  

1

1

1

1

2

Ubiquinone and other terpenoid-quinone biosynthesis

7.4

2.7E-02

1

  

1

   

1

Valine, leucine and isoleucine biosynthesis

5.3

4.8E-02

     

2

  

Valine, leucine and isoleucine degradation

3.8

3.6E-02

1

   

1

1

2

 

Vitamin B6 metabolism

10.5

1.3E-02

 

2

      
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