Figure 10
From: Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors
Auto-FPFA characterization of GCaMP6s, an intensity based calcium biosensor. Auto-FPFA was used to measure count rate (a), <N> (b), brightness (c), τD (d), fraction dark state (e), τF (f), steady state anisotropy (g), and average lifetime (e) of two different homogenates prepared from cells expressing GCaMP6s. Homogenates were diluted into buffers with defined calcium concentrations. The homogenate concentration was six times as concentrated for samples at or below 250 nM calcium as those used at higher calcium concentrations. Each point is the mean ± SD (N = 4, two from each homogenate preparations). Dashed lines are a four parameter, variable slope dose-response fit in all panels except for panels e and f where the dashed lines simply connect the three data points.
