Figure 2
From: Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors
Auto-FPFA acquisition software control and software/hardware interface schematic. Left Panel: Diagram illustrates the Lab View control algorithm, and its integration with B&H SPCM software algorithm. LabView was used as the ‘master’ control data acquisition software, while B&H SPCM software was ‘slaved’ to it. LabView specifically controlled the XY-dual axis scanning stage controller that sequentially steps through the 96 samples in a micro-titer dish, as well as the Z-axis stage controller used for the auto-focus function. The ‘slaved’ B&H SPCM software was used to control the TCSPC SPC-130-EM card for data acquisition and data storage. Right Panel: Diagram illustrates the auto-FPFA software/hardware interface. The central component of this system is the B&H SPC 130-EM TCSPC card. Both LabView and B&H SPCM software communicate (send commands and receive data) with the TCSPC card via a B&H SPC driver. Once activated, the TCSPC card receives data from the two HPM-100-40 hybrid photon detectors via a HRT 41 multiplexing router. The TCSPC card also receives laser pulse timing data from a photo-diode detector (not shown, but see Fig. 1).
