Figure 7
From: Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors
Auto-FPFA characterization of Venus-Twitch4-Venus, a homo-FRET based calcium biosensor. Auto-FPFA was used to measure count rate (a), <N> (b), brightness (c), diffusion time (τD, d), steady state anisotropy (e), and average lifetime (f) of two different homogenates prepared from cells expressing Venus-Twitch4-Venus. Homogenates were diluted into buffers with defined calcium concentrations. Each point is the mean ± SD (N = 4, two from each homogenate preparations). Dashed lines are: a linear fit (panels a, b, c and f), a four parameter, variable slope dose-response fit (panel d) or a biphasic dose-response fit (panel e).
