Figure 8
From: Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors
Auto-FPFA characterization of Venus monomers, a control for non-specific calcium changes in Venus photo-physics. Auto-FPFA was used to measure count rate (a), <N> (b), brightness (c), diffusion time (τD, d), steady state anisotropy (e), and average lifetime (f) of two different homogenates prepared from cells expressing Venus. Homogenates were diluted into buffers with defined calcium concentrations. Each point is the mean ± SD (N = 4, two from each homogenate preparations). Dashed lines are a linear fit.
