Figure 1
From: Hepatic stellate cells secrete Ccl5 to induce hepatocyte steatosis
Mouse model of steatohepatitis using choline-deficient, L-amino acid-defined, high fat diet. (A–C) After three weeks of CDAHFD, mice were both grossly and microscopically affected with steatohepatitis that resembles early NASH. (D) Hepatic stellate cells harvested from CDAHFD fed mice retained retinoid positive lipid droplets that fluoresce with ultraviolet light. Nearly 99% of the isolated hepatic stellate cell population fluoresced with ultraviolet light based on FACS analysis. (E) The harvested hepatic stellate cell population did not express albumin or F4/80 based on immunofluorescence. CDAHFD, L-amino acid-defined, high fat diet; NASH, non-alcoholic steatohepatitis; UV, ultraviolet.
