Figure 3
From: Hepatic stellate cells secrete Ccl5 to induce hepatocyte steatosis
Hepatic stellate cells isolated from mice with steatohepatitis induce steatosis and inflammation in cocultured hepatocytes. (A) Diagram of the experiment showing coculture of healthy hepatocytes with hepatic stellate cells from either control mice or from mice with CDAHFD induced steatohepatitis. (B) Top row: Healthy hepatocytes cocultured with HSCs from control mice showed very few lipid droplets stained with Bodipy. Bottom row: Initially healthy hepatocytes cocultured with HSCs from mice with CDAHFD induced steatohepatitis developed significantly more lipid droplets. (C) Induction of fatty accumulation in hepatocytes can be achieved when the conditioned media from HSCs from mice with CDAHFD induced steatohepatitis is applied to healthy hepatocytes. (D) Top row: When quiescent HSC conditioned media was applied to healthy hepatocytes, steatosis was not induced. Bottom row: Conditioned media of HSCs from mice with CDAHFD induced steatohepatitis induced steatosis in initially healthy hepatocytes. Lipid droplets stained with Bodipy. (E) Initially healthy hepatocytes cocultured with HSCs from mice with CDAHFD induced steatohepatitis expressed higher levels of several inflammatory cytokines and chemoattractants than when they were cocultured with control HSCs, measured with qPCR. Data are presented as mean +/− SD (*P < 0.05). NASH, non-alcoholic steatohepatitis; CDAHFD, choline-deficient L-amino acid defined high fat diet; Hep, hepatocyte; HSC, hepatic stellate cell.
