Figure 1 | Scientific Reports

Figure 1

From: Roles of Enhancer RNAs in RANKL-induced Osteoclast Differentiation Identified by Genome-wide Cap-analysis of Gene Expression using CRISPR/Cas9

Figure 1

Cap-analysis of gene expression (CAGE) analyses of transcripts during osteoclast differentiation. (a) Schematic of the methodology for osteoclast differentiation. (b) Pairwise scatter plot of CAGE tag counts per cluster transcription start site (CTSS) and correlations between all possible pairs of samples (four RANKL-stimulated samples and four control samples). Numbers in the boxes represent the values of the correlation coefficients. (c) Summary of the pipeline of CAGE transcriptomes for identification of RANKL-induced genes. Among nearly 3 million CTSSs, 132,744 sites were identified as CAGE-detected transcription start sites (TSSs), and 6,933 and 6,413 sites were defined as increased or decreased TSSs, respectively, by differential expression analyses. (d) Comparison of the distances between identified TSSs and known promoters. (e) Pie charts representing genomic annotations of increased and decreased TSSs.

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