Table 1 List of candidate eRNA regions in response to RANKL stimulation.

From: Roles of Enhancer RNAs in RANKL-induced Osteoclast Differentiation Identified by Genome-wide Cap-analysis of Gene Expression using CRISPR/Cas9

SYMBOL

aannotation

gene strand

bdistance to TSS

FDR

cpermissive enhancer (FANTOM5)

+strand

−strand

Sbno2

Promoter (2–3 kb)

−2769

8.93.E-09

9.12.E-59

Jdp2

3′ UTR

+

38448

2.08.E-58

2.09.E-08

Nrp2

Distal Intergenic

+

−11851

2.78.E-03

3.39.E-42

Egr2

Promoter (2–3 kb)

+

−2178

1.59.E-19

4.39.E-02

Nfatc1

Intron

51979

8.21.E-10

2.92.E-14

+

Dcstamp

Distal Intergenic

+

−8165

4.62.E-12

3.90.E-03

+

Zbtb7a

Distal Intergenic

+

−7039

4.80.E-12

1.19.E-03

Fosl2

Intron

+

−83707

2.00.E-07

3.40.E-10

+

Zbtb7a

Intron

+

5049

1.66.E-08

1.31.E-03

Nrp2

Distal Intergenic

+

−7217

3.64.E-08

1.44.E-02

Rap1b

Distal Intergenic

−21278

2.00.E-07

1.94.E-04

+

Acp5

Promoter (<=1 kb)

370

5.44.E-07

6.07.E-02

Fos

Distal Intergenic

+

36547

3.33.E-04

2.14.E-05

Sema4d

Promoter (<=1 kb)

579

6.92.E-05

2.11.E-02

+

Rnf19b

Intron

+

9719

3.50.E-04

1.88.E-02

Nrp2

Distal Intergenic

+

−24014

1.01.E-03

3.62.E-02

Ltbp1

Intron

+

93668

2.11.E-02

4.15.E-02

Fos

Distal Intergenic

+

12780

3.22.E-02

3.91.E-02

  1. aAnnotation of gene segments defined by edgeR.
  2. bThe distance to the transcription start site (TSS) measured from the TSS of protein-coding measured by edgeR.
  3. cThe enhancer regions were classified into robust and permissive, based on the read counts. The permissive enhancer was defined by FANTOM5 mouse permissive enhancer phase 1 and 223.