Figure 7

GM-CSF enhances the CAWS-induced inflammatory cytokine production in BMMs BMMs were pretreated with or without IL-10 (40 ng/mL) for 1 h, and then treated with CAWS (10 μg/mL) and/or GM-CSF (1 ng/mL) for 48 h. (A) The TNF-α and IL-6 protein levels in the supernatants were assessed (n = 3). (B) The mRNA levels of Clec4n were assessed by a real-time RT-PCR analysis (n = 3). Data are expressed as the mean ± SEM. *p < 0.05. (C) BMMs were treated with or without GM-CSF (1 ng/mL) for 48 h. The cells were pretreated with or without IL-10 (40 ng/mL) for 1 h, and then treated CAWS (10 μg/mL) for the indicated periods. Cell lysates were prepared and analyzed by Western blotting with antibodies against Syk, p-Syk, ERK1/2, p-ERK1/2, and β-actin.