Figure 9

Confocal microscopy visualization of PS exposure after 20 min Pb²⁺ incubation. Annexin V was used to detect PS translocation and detected by fluorescence (green). Transmitted light (A), fluorescence (B) and mix of both images (C). Percentage of cells that expose PS using FACS (three different experiments, 10000 cells per experiment) (D). RBC treated with both annexin V and antiCer (Alexa Fluor 633 as secondary antibody) (E). Images in C and E are a combination of transmitted light and fluorescence confocal microscopy, where red dots indicate ceramide-enriched zones detected by the antiCer antibody as in Fig. 8.