Table 4 Standards and conditions for mass spectrometric analysis of RBC sphingolipids.

From: Pb(II) Induces Scramblase Activation and Ceramide-Domain Generation in Red Blood Cells

Lipid Class

Standard

Polarity

Mode

m/z ion

Collision Energy (eV)

Ceramide

C17Cer

+

Product ion

264.34

25

Dihydroceramide

C17Cer

+

Product ion

266.34

25

Hexosylceramide

C8GC

+

Product ion

264.4

30

Hexosyldihydroceramide

C8GC

+

Product ion

266.4

30

Sphingomyelin

C12SM

+

Product ion

184.07

26