Figure 1
From: GmBEHL1, a BES1/BZR1 family protein, negatively regulates soybean nodulation
NNC1 interacts directly with GmBEHL1. (a) Results of Y2H assay to detect interactions between NNC1 and GmBEHL1. Yeast cells co-transformed with the constructs pGADT7/pGBKT7-GmBEHL1, pGADT7-NNC1/pGBKT7-GmBEHL1, and pGADT7-NNC1/pGBKT7 were selected and grown on selective media lacking Leu, and Trp (SD/−2); surviving cells were subsequently transferred to selective media lacking Ade, His, Leu, and Trp (SD/−4) to test for protein-protein interactions. (b) Results of a BiFC assay to detect the interaction of NNC1 with GmBEHL1. GmBEHL1 and NNC1 were fused to the N-terminus of YFP (nYFP) and C-terminus of YFP (cYFP), respectively. Bars = 25 μm.
