Figure 3

Alterations in GmBEHL1 expression affect the transcript levels of nodulation-related genes. (a) qRT-PCR analysis of ENOD40, GmNSP1, GmNSP2, GmNIN, pre-miR172c, and GmRIC1 in roots transformed with EV1 and 35S:GmBEHL1 at 2 DAI (n = 6). (b) qRT-PCR analysis of ENOD40, GmNSP1, GmNSP2, GmNIN, pre-miR172c, and GmRIC1 in roots transformed with EV2 and amiR-GmBEHL1 at 2 DAI (n = 6). We set all of the transcript profiles of the GmENOD40, GmNSP1, GmNSP2 and GmNINa in 2 DAI EV hairy roots as “1”. The transcript amounts in each sample were normalized to those of ELF1b. The expression levels shown are the means ± SDs from three replicates. Asterisks represent statistically significant differences compared to the empty control (Student’s t-test; *p < 0.05, **p < 0.01, and ***p < 0.001).