Figure 7
Transcriptional expression analysis of pGDNF in postnatal porcine testes. (A) Quantitative gene expression analysis of GDNF, DDX4, ZBTB16 and GFRA1 in prepubertal porcine testes. A total of 29 testes (7 from 0–1 weeks, 9 from 7–8 weeks and 13 from 14–16 weeks) were analysed for relative gene expression of the indicated genes by qRT-PCR. Values were normalised to the 18S ribosomal RNA expression levels. Data are presented as mean ± SEM. *P < 0.05. (B) Simple regression analysis between DDX4 expression and GDNF or ZBTB16 expression in individual testis samples. A negative correlation was found between DDX4 and GDNF (left), whereas a strong positive correlation was found between DDX4 and ZBTB16 (right).
