Figure 3

Cytotoxicity assay of the formulations in fibroblasts 3T3 (A) and lung cells (V79) (B). Cells were treated with the different formulations Tween 80, control nanoparticles (NP), CVC and LNL emulsified (C + L), and nanoparticles containing CVC and LNL co-loaded (NP_C + L) for a period of 24 hours at 37 °C. Cell viability was assessed by the MTT assay, with the control group being considered 100% of cell viability. Values represent the mean ± SD of three determinations (n = 9).