Figure 6 | Scientific Reports

Figure 6

From: Amelioration of Experimental autoimmune encephalomyelitis and DSS induced colitis by NTG-A-009 through the inhibition of Th1 and Th17 cells differentiation

Figure 6

NTG-A-009 inhibits Th1 and Th17 cells in vivo and attenuate DSS induced colitis. C57BL/6 mice were orally administered with 2.5% (w/v) DSS in drinking water for 7 days to induce colitis. NTG-A-009 was orally administered every day (n = 6 mice in each group). (a) Body weight of water, DSS and NTG-A-009 treated mice was recorded daily and presented as percentage of original body weight. (b) Colon length of mice treated with water, DSS and NTG-A-009. (c) CD4+ and CD8+ T cells infiltration was detected from the Lymphocytes isolated from colonic lamina propria of water, DSS and NTG-A-009 treated group and analyzed by FACS. (d) The percentage of Th1 and Th17 cells was determined by the stimulation of splenocytes from each group with PMA, ionomycin and golgistop for 4 hour and analyzed by FACS through intracellular staining of IFN-γ and IL-17. (e) H&E staining of colonic section with histological score (magnification 20X). Data represent three independent experiments. Two-way anova (A) or *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page