Figure 6
From: RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection
Real-time detection of GFP mRNA present in total RNA isolated from E. coli carried pET-AcGFP with induction. The amounts of total RNA used were as follows: open circles, 80 ng; open triangles, 40 ng; open diamonds, 20 ng; open squares, 10 ng; closed circles, 2 ng; and closed triangles, 0 ng. RNase H was used at 0.03 units per reaction. All reactions were performed in triplicate, and the mean and error were calculated.
