Figure 5

Pupation of L. japonica induces expression of genes involved in lipid metabolism. Total RNA obtained from both larvae and pupae was purified for RNA-seq. Differential mRNA expression between samples was determined with Fold change and p-value. (A) Log2 fold changes in RefSeq gene bodies in larvae and pupae and the corresponding significance values displayed as fold change >2 or <0.5 and p value < 0.05. The x-axis represents −log10 transformed significance, and the y-axis represents log2 transformed fold change. Red points represent up-regulated DEG. Blue points represent down-regulated DEG. Black points represent non-DEGs. Pk, pyruvate kinase; cs, citrate synthase; tgl, triacylglycerol lipase; gapdh, glyceraldehyde-3-phosphate dehydrogenase; tim, triosephosphate isomerase. (B) Relative expression levels of genes from the RNA-seq data of J and Y. Data is a representative set (three) containing duplicate samples. Significance levels: ***Fold change >2 or <0.5 and p value < 0.001. (D,E) Heat map representation of gene expression changes for significantly enriched metabolic KEGG pathways during pupation (glycerophospholipid metabolism, (C) glycerolipid metabolism, (D) fat digestion and absorption (E). The log10 expression values for each sample were clustered and uniformed in rows. Tcjhe, juvenile hormone esterase; LPCAT1, lysophosphatidylcholine acyltransferase; CG8804, similar to CG8804-PA; dgkA, diacylglycerol kinase; Lipf, lipase; BSSL, bile salt-stimulated lipase; PNLIP, pancreatic triacylglycerol lipase; Pnpla3,patatin-like phospholipase domain containing 3;CES1, carboxylesterase 1;DAK, bifunctional ATP-dependent dihydroxyacetone kinase;Rga, regulator of gene activity protein;BCHE, cholinesterase;CEL, esterase FE4-like; ALDH3A2, aldehyde dehydrogenase family 3 member A2; ALDH7A1,aldehyde dehydrogenase family 7 member A1; EXOSC10,exosome component 10; AGK,acylglycerol kinase; AKR1,aldo-keto reductase 1; RIT1, tRNA A64-2′-O-ribosylphosphate transferase; ABCG5,ATP-binding cassette sub-family G member 5; SLC27A4,solute carrier family 27; FABP1,fatty acid-binding protein 1; ACAT2,acetyl-CoA C-acetyltransferase; Pla2g3,phospholipase A2, group III;CG3009, CG3009 gene product from transcript CG3009-RA; Scarb1, scavenger receptor class B member 1; GOT2, aspartate aminotransferase.