Figure 4 | Scientific Reports

Figure 4

From: Treatment of T1D via optimized expansion of antigen-specific Tregs induced by IL-2/anti-IL-2 monoclonal antibody complexes and peptide/MHC tetramers

Figure 4

Combined Optimized treatment improves the expansion of 2.5 mi+ Treg cells and controls the increase of antigen-specific Foxp3− T cells in NOD.Foxp3EGFP mice in pancreatic islets. The same animals used in Fig. 3 C–E were used for the analysis of 2.5 mi+ T cells in pancreatic islets. (A) Left, representative dot plots depicting Ag-specific Tconv and Treg percentages ± SEM within total CD4+ T cells in islets. Right, bar diagram showing the ratio of Tconv/Treg in 2.5 mi+ T cells. (B) Percentages of activated (CD25hi) antigen-specific Tconv (Foxp3−) and Treg (Foxp3+) cells within the 2.5 mi+ T cell population in islets. Error bars in graphs indicate mean ± SEM of 3 to 4 independent experiments including 2-3 animals per group. The p values result from a Student t test analysis. **p < 0.005, ***p < 0.0005. No Ag7/GPI-specific T cell expansion was observed.

Back to article page