Figure 3 | Scientific Reports

Figure 3

From: Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology

Figure 3

Exploration of protein levels in CSF, plasma and brain of ischemic and sham-control animals. Plots of protein abundance data (RFU: relative fluorescent units) in (A) CSF samples obtained pre- and post-MCAO and (B) plasma samples obtained 2 h after MCAO or sham-control surgery (n = 6 samples per experimental group for all cases). (C) Plot of protein abundance (relative quantification, ratio IP vs. CL hemisphere of each animal) in brain homogenates samples obtained 2 h after MCAO or sham-control surgery (n = 4 samples for sham; n = 8 samples for MCAO). In all cases, IP and CL samples from each animal are run in the same gel and individually corrected by their respective β-actin loading controls (Supplemental Figure S3). (D) Representative Western Blots signals used for quantification of proteins in brain. Bars indicate mean ± SD. For all graphs, *p < 0.05 and #p < 0.1. Abbreviations: IP: ipsilateral hemisphere; CL: contralateral hemisphere.

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