Figure 4 | Scientific Reports

Figure 4

From: Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases

Figure 4

The range of relative strain activity and extent of amino acid residue mutations in positive variants. (a) The ratios of positive variants with different relative strain activity. A, B, C, D, E, F, G, H, I, J were the ratios of positive variants with 1 to 1.05, 1.05 to 1.10, 1.10 to 1.15, 1.15 to 1.20, 1.20 to 1.25, 1.25 to 1.30, 1.30 to 1.35, 1.35 to 1.40, 1.40 to 1.50, 1.50 to 1.82 fold strain activity than that of wild-type. (b) The ratios of positive variants with different amino acid residue mutations. The ratios of positive variants with different amino acid residues higher than 5% among the positive variants from two round of diversification and screening.

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