Table 1 Primers used in this study. The underlined text indicates the restriction sites. The bold type of R1 indicates the His-tag gene. The sites of the variations are shown in lowercase letters.
Primers | Sequences 5′-3′ |
|---|---|
F1 | CGGGATCCATGAGACTGCTGATCCTGG |
R1 | GCACGCGTTTAGTGGTGGTGGTGGTGGTGCGCGGATTTCCCAATTTCG |
F2: S17G | AGTGTGCCTggcGAAGAGGGATACGAATCATCACTGT |
R2:S17G | TCTTCgccAGGCACACTTGCGATTGTTCCTCC |
F3:A90S | CGCTTACACAagtTCGATGCTTAGCTTTATGGTGAGA |
R3: A90S | TCGAactTGTGTAAGCGAGCGTGTCTGTACCG |
F4: R156S | CAAGGTCagtGCAGTTGGTCTTAACGCCTTTC |
R4: R156S | CAACTGCactGACCTTGCTTACTCTCACTCCGA |
F5: K272A | CTTGACCgccTACAAAGTCGGCCGGAAAGCGT |
R5: K272A | CTTTGTAggcGGTCAAGTCAACGCCGTCATAT |
