Table 2 Purification of recombinant L-asparaginase.
Purification step | Volume (mL) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|
Crude enzyme | 5 | 23.87 ± 1.3 | 699.75 ± 3.3 | 29.31 ± 1.84 | 1 | 100 |
Ni2+-affinity chromatography | 3 | 0.2873 ± 0.01 | 426.3 ± 2.8 | 1483.81 ± 63.61 | 50.62 ± 5.6 | 60.92 ± 0.7 |
