Figure 2 | Scientific Reports

Figure 2

From: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape

Figure 2

C151 in KEAP1 is the primary sensor for TBE-31 in MEF cells. Western blot analyses of total cell lysates of KEAP1-knockout MEF cells rescued with either wild-type (WT) (A,B), single cysteine mutant C151S (A), double cysteine mutant C273W/C288E (B), or triple cysteine mutant C151S/C273W/C288E (A and B) of mouse N-terminally tagged HA-KEAP1. Cells (3 × 105 per well), growing in 6-well plates, were exposed to vehicle (0.1% DMSO) or TBE-31 for 3 h, after which the cells were lysed. Immunoblotting was performed on cell lysates using antibodies raised against NRF2, HA and α-tubulin.

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