Figure 7
C151 in KEAP1 is required for the ability of TBE-31 to inhibit human IL6-luciferase reporter gene expression in primary murine peritoneal macrophages challenged with LPS. (A) Primary peritoneal macrophages were isolated from KEAP1+/+::hIL6-Luc or KEAP1C151S/C151S::hIL6-Luc mice and the cells (n = 3) were treated ex vivo with LPS (1 ng/ml) and/or TBE-31 (10 nM) for 4 h. (A) The luciferase reporter activity was determined in total cell lysates. *p < 0.05.
