Figure 2
From: HDAC1 inhibition by MS-275 in mesothelial cells limits cellular invasion and promotes MMT reversal
Treatment with MS-275 promotes nuclear histone H3 acetylation. (A) Confocal immunofluorescence analysis of MCs treated with DMSO, MS-275 (250 nM), MC-2500 (250 nM) for 12 h. Cells were fixed, permeabilized and stained with anti-acetylated histone H3 (yellow). Images were acquired by confocal microscopy. Nuclei were stained with Hoechst 33342 (blue). Data are representative of three independent experiments. (B) Quantification of the experiment shown in (A). The histogram shows fluorescence intensities of nuclear acetylated histone H3 staining quantified using the software ImageJ. Bars represent ± SEM. At least 400 nuclei were analyzed per condition. P < 0.05 was considered significant.
