Figure 2

DROSHA reduction contributes to miRNA dysregulation in SMA motor neurons. (A) Successful Drosha knockdown (KD) was achieved with combining two different Drosha siRNAs. siRNAs were transfected to 3DIV motor neurons and KD was confirmed after 72 hours: n = 3 (B) mRNAs levels of Drosha and Dgcr8 were measured by qRT-PCR in Drosha KD motor neurons: n = 10 (C) miRNA levels in 6DIV motor neurons: n = 12 (WT and SMA, except miR-10a-5p and miR-10b-5p), n = 16 (WT and SMA, miR-10a-5p and miR-10b-5p) (D) Pie charts represent the composition of miRNAs in 6DIV motor neurons. miRNAs account for less than 1% of total reads were grouped as other miRs. (E) Venn diagram shows that 37 common miRNAs were down regulated in SMA and Drosha KD neurons compared their controls. Data are represented as mean±SEM, Statistical significance is determined with t-test, *p < 0.05, **p < 0.01 and ***p < 0.001.