Figure 5
From: Neuronal activity regulates DROSHA via autophagy in spinal muscular atrophy
Neuronal activity regulates autophagy and DROSHA. (A) Western blot analysis of DROSHA, LC3-I, LC3-II, p62 levels in 10DIV WT motor neurons after 55 mM KCl induced depolarization at different time points. (B) Quantification of DROSHA: n = 3 (C) Quantification of p62: n = 6, (D–F) Quantification of LC3-I and LC3-II, and ratio between LC3-II and LC3-I: n = 6. Data are represented as mean ± SEM. Statistical significance is determined with t-test. *p < 0.05, **p < 0.01 and *** p < 0.001.
