Figure 6
From: Neuronal activity regulates DROSHA via autophagy in spinal muscular atrophy
DROSHA is localized in endosomal-autophagy compartments in stimulated and SMA motor neurons. Subcellular localization of DROSHA was checked with vesicular markers. WT neurons were stimulated with 55 mM KCl or 100μM NMDA for 1 hour. SMA neurons were not stimulated. (A,C,E) Images show co-localization of DROSHA with vesicular markers. Arrows point overlapped signals. green: DROSHA, red: vesicular organelle markers, listed in the figure. *Overlapped signals. (B,D,F) Percentage of neurons showing co-localization of DROSHA with vesicular markers. Neurons are individually analyzed. At least 3 independent biological samples were analyzed and 136 to 275 neurons were analyzed per group. Data are represented as mean ± SEM. Statistical significance is determined with t-test. *p < 0.05 and **p < 0.01.
