Figure 7
From: Neuronal activity regulates DROSHA via autophagy in spinal muscular atrophy
DROSHA reduction promotes neurites outgrowth. (A) Efficiency of two different shRNA sequences are tested. Plasmids were transfected in 1DIV and images were taken in 6DIV. DROSHA intensity from shRNA transfected neurons were compared to one from non-transfected neighboring neurons. GFP was used as a marker of transfection. (B) Bar graphs represent efficiency of shRNAs against Drosha. For shDROSHA-1, 18 transfected neurons are compared to 70 non-transfected neurons. For shDROSHA-2, 12 transfected neurons are compared to 42 non-transfected neurons. (C) Morphology of control and DROSHA knockdown motor neurons are visualized with GFP, and length of neurites was measured. Analyzed neurons were from 9 different transfections (from at least 3 independent biological samples). 36 neurons were analyzed per group. Analyzed neurons are all confirmed as ChAT positive (ChAT: Choline acetyltransferase, motor neuron marker). Data are represented as mean±SEM. Statistical significance is determined with t-test. *p < 0.05, **p < 0.01 and ***p < 0.001. (D) A schematic model for neuronal activity-autophagy-DROSHA-miRNA pathways in motor neurons.
