Figure 1

Expression of HBV-mRNAs and intracellular core-associated HBV-DNA measured by real-time PCR following the transfection of full-length linear monomers of specific HBV/D-subgenotype, D1, D2, D3 and D5 into Huh7 cells. Relative expression of (A) pregenomic-RNA (pgRNA), (B) preC-mRNA, (C) ratio of PreS2/PreS1 mRNA, (D) HBx mRNA, (E) level of intracellular core-associated HBV-DNA among different HBV/D-subgenotypes. Paired t-test p values; *p < 0.05, **p < 0.01, ***p < 0.001.