Figure 4
(A) Survival curve of infected mice after inoculation with whole, live bacteria. Similar bacterial loads of “M. shinshuense” (ShT-P or ShT-N) or M. ulcerans (MU-4 or MU-8) were inoculated into the footpads of BALB/c mice (n = 6, each group). The mice inoculated with ShT-P died within 5 weeks of inoculation (middle, right panels) whereas those inoculated with ShT-N were all alive even after 8 weeks (middle panel). (B) Bacterial load in hind footpads of mice inoculated with “M. shinshuense” or M. ulcerans at entry, 1 day and 8 weeks after inoculation or at the time of death. Bacterial load is shown as log10 CFU/foot pad. ShT-N CFUs were absent after 8 weeks. (C) Body weight of infected mice or control mice after inoculation with bacteria. Body weight of infected ShT-P and MU-4 was significantly increased relative to that of control animals (p < 0.01). (D) Degree of erythema in the hind footpads of the inoculated mice. 1+ : slight, 2+ : moderate, 3+ : severe. (E) Measurement of hind footpad swelling induced by “M. shinshuense” or M. ulcerans.
