Table 1 Characteristics of bacteria tested in this study.

From: Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic

 

“M. shinshuense”

M. ulcerans

ATCC 33728

MU-4

MU-8

ShT-P (pigmented)

ShT-N (non-pigmented)

Sh-753

Mycolactone production

+

+

+

Colony:

 Color (pigmentation)

+

+

+

 Morphology

R

R

R

R

R

Growth on 2% Ogawa medium slant

 14 days

+

+

+

 30 days

+

+

+

+

+

Growth at:

 25 °C

+

+

+

+

+

 32 °C

+

+

+

+

+

 37 °C

 42 °C

Growth on 500 μg/ml p-nitrobenzoic acid

Growth on NaCl medium (5%)

Tween 80 hydrolysis

Nitrate reduction

Arylsulfatase

Pyrazinamidase

Iron uptake

MPB64 Ag production

Semi−quantitative catalase

+

+

+

+

+

68 °C catalase

+

+

+

+

+

Urease

+

+

+

  1. R: rough. See Fig. 2 for visualization of mycolactone production by TLC.