Table 2 PCR detection of eight giant plasmid-associated genes in M. ulcerans or “M. shinshuense” strains.

From: Naturally occurring a loss of a giant plasmid from Mycobacterium ulcerans subsp. shinshuense makes it non-pathogenic

 

pMUM001 marker genes

repA

parA

MUP011 (STPK)

mls

mlsAT

MUP038 (TEII)

MUP045 (KSIII)

MUP053 (p450)

ShT-P

+

+

−

+

+

+

+

+

ShT-N

−

−

−

−

−

−

−

−

Sh-753

+

+

−

+

+

+

+

+

MU-4

+

+

+

+

+

+

+

+

MU-1615

+

+

+

+

+

+

+

+

MU-8

+

+

−

+

−

−

+

+

  1. ShT-P: ATCC 33728, pigmented strain; ShT-N: ATCC 33728, non-pigmented strain; Sh-753: “M. shinshuense”, virulent; MU-4: M. ulcerans 97–107 African strain, virulent; MU-1615: M. ulcerans Malaysian strain, virulent; MU-8: M. ulcerans 5143 Mexican strain, avirulent; STPK, serine/threonine protein kinase gene; mlsAT, acyltransferase domain of mls; TEII, type II thioesterase gene; KSIII, type III ketosynthase gene; p450, p450 hydroxylase gene.
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