Figure 4
From: Transcriptomic response of breast cancer cells to anacardic acid

AnAc inhibits TNFα-induced NFκB luciferase reporter activity in transiently transfected MCF-7 cells. MCF-7 cells were transfected with a NFκB response element luciferase reporter and a Renilla reporter for 48 h. Cells were treated with 10 ng/ml TNFα EtOH (vehicle control, open bar, and 1–40 µM AnAc for 6 h before performing dual luciferase assay. Values are the average of three separate wells in one experiment ± SEM. *p < 0.01 versus EtOH control (open bar).