Table 5 Genes uniquely upregulated by anacardic acid in MCF-7 cells after 6 h of treatment with 13.5 µM.

CELSR3

0.10084

8.2254

5.00E-05

Cadherin, EGF LAG Seven-Pass G-Type Receptor 3, a GPCR

GO:0007413:axonal fascicula-tion: GO:0032880: regulation of protein localization: GO: 0007166: cell surface receptor signaling pathway

CELSR3 is a multi-pass membrane protein. CELSR3 is methylated in neuroendocrine¹¹⁰ and oral tumors¹¹¹.

CACNG8

3.82558

9.87498

5.00E-05

Calcium Channel, Voltage-Dependent, Gamma Subunit 8

GO:0030666:endocytic vesicle membrane; GO:2000311: regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity;

CACNG8 is a transmembrane voltage-gated calcium channel subunit regulatory protein.

FAM73A

0.45163

1.4673

0.00045

Family With Sequence Similarity 73

GO:0016021; integral to membrane

FAM73 (Mitoguardin-1, MIGA1) is a regulator of mitochondrial fusion.

NTRK3

0.47030

1.2800

0.0039

Neurotrophic Receptor Tyrosine Kinase 3

GO:0048691:positive regulation of axon extension; GO:0038179: neurotrophin signaling; GO:0019056: modulation by virus of host transcription

NTRK3 is a membrane bound receptor that binds neurotrophin and activates MAPK signaling.

LEKR1

0.59333

1.64146

0.0044

Leucine, Glutamate And Lysine Rich 1

GO:0005840:ribosome

GO:0006412:translation

GO:0003735:structural constituent of ribosome

A missense mutation in the LEKR1 gene was associated with epithelial ovarian cancer risk¹¹².

HIST1H1D

0.74898

1.6380

0.0051

Histone Cluster 1, H1d

GO:0000790:nuclear chromatin

GO:0006334:nucleosome assembly; GO:0000786: nucleosome

nucleosomal linker histone increased after PPM1D knockdown ZR-75-1 breast cancer cells¹¹³.

VIM

105.535

166.93

0.0061

Vimentin

GO:0006921:cellular component disassembly involved in apoptosis; GO:0060020: Bergmann glial cell differentiation; GO:0045109: intermediate filament organization

RN7SL2

0.24437

2.3353

0.00655

RNA, 7SL, Cytoplasmic 2

RNA component of the signal recognition particle, a cytoplasmic ribonucleo-protein complex

ARHGEF26-AS1

53.1195

76.392

0.0079

ARHGEF26 Antisense RNA1

Non-coding RNA

RABGGTB

0.49542

2.43989

0.00925

Rab Geranylgeranyltransferase Beta Subunit

GO:0018344:protein geranylgeranylation

GO:0004663:Rab geranyl-geranyltransferase activity; GO:0006464:cellular protein modification process

RABGGTB is the beta-subunit of the enzyme Rab geranylgeranyl-transferase (RabGGTase).

TMC5

1.43847

0.0121

Transmembrane Channel-Like 5

GO:0016021:integral to membrane; GO:0006811:ion transport

A multi-pass membrane protein inhibited by E₂ in MCF-7 cells⁵³.

RPS4XP16

1.97726

3.21612

0.013

Ribosomal Protein S4X Pseudogene 16

ETV6

0.94319

1.5434

0.0285

Ets Variant, TEL1 oncogene, transcription factor

GO:0043565:sequence-specific DNA binding; GO:0006355: regulation of transcription, DNA-dependent; GO:0019904: protein domain specific binding

ETV6 interacts with corepressors: NCOR and SMRT (reviewed in¹¹⁴). ETV6 is downstream of JNK (Supplementary Fig. 7).

MAPK10

0.43154

1.19202

0.0399

Mitogen-Activated Protein Kinase 10

GO:0007258:JUN phosphorylation; GO:0034146: TLR5 signaling pathway; GO:0038124: TLR6:TLR2 signaling pathway

MAPK10 is a proapoptotic protein^115,116. Higher in luminal breast cancer cell lines was associated with sensitivity to everolimus¹¹⁷

HIST1H2AK

0.04925

Histone Cluster 1, H2ak

GO:0006334:nucleosome assembly; GO:0000786: nucleosome; GO:0019899: enzyme binding

E₂ inhibits HIST1H2AK expression in MCF-7 cells⁵³.