Table 1 Strains used in the study.
From: Penetrating cations induce pleiotropic drug resistance in yeast
Strain | Genotype | Parental strains and/or references |
|---|---|---|
W303-1A | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 | Laboratory of A. Hyman |
Δpdr1 a | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 pdr1Δ::KanMX4 | W303-1A |
Δpdr3 a | MATalpha ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 pdr3Δ::KanMX4 | W303-1B |
Δpdr1Δpdr3 b | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 pdr1Δ::KanMX4 pdr3Δ::KanMX4 | Δpdr1, Δpdr3 |
Δrtg1 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 rtg1Δ::KanMX4 | Zyrina et al.33 |
Δrtg2 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 rtg2Δ::KanMX4 | Zyrina et al.33 |
Δrtg3 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 rtg3Δ::KanMX4 | Zyrina et al.33 |
Δyap1 | MATalpha ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 yap1Δ::KanMX4 | Zyrina et al.33 |
Δrtg2Δyap1 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 rtg2Δ::KanMX4 yap1Δ::KanMX4 | Zyrina et al.33 |
Pdr5-GFP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PDR5-GFP::HIS3 | Huh et al.49 |
Snq2-GFP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SNQ2-GFP::HIS3 | Huh et al.49 |
Snq2-GFP Δpdr1Δpdr3 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 SNQ2-GFP::HIS3 pdr1Δ::KanMX4 pdr3Δ::KanMX4 | SNQ2-GFP, Δpdr1Δpdr3 |
Snq2-GFP Δpdr1 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 SNQ2-GFP::HIS3 pdr1Δ::KanMX4 | SNQ2-GFP, Δpdr1Δpdr3 |
Snq2-GFP Δpdr3 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 SNQ2-GFP::HIS3 pdr3Δ::KanMX4 | SNQ2-GFP, Δpdr1Δpdr3 |
P GAL -SNQ2 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SNQ2::HIS3 | Knorre et al.26 |
P GAL -PDR5 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-PDR5::HIS3 | Knorre et al.26 |
Pdr5-GFP Δpdr1Δpdr3 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 PDR5-GFP::HIS3 pdr1Δ::KanMX4 pdr3Δ::KanMX4 | Pdr5-GFP, Δpdr1Δpdr3 |
Pdr5-GFP Δpdr1 a | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PDR5-GFP::HIS3 pdr1Δ::KanMX4 | Pdr5-GFP |
Pdr5-GFP Δpdr3 a | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PDR5-GFP::HIS3 pdr3Δ::KanMX4 | Pdr5-GFP |
Pdr5-GFP Δrtg2 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 PDR5-GFP::HIS3 rtg2Δ::KanMX4 | Pdr5-GFP, Δrtg2 |
Pdr5-GFP Δyap1 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 PDR5-GFP::HIS3 yap1Δ::KanMX4 | Pdr5-GFP, Δyap1 |
Pdr5-GFP Δrtg2Δyap1 b | MATa ade2-101 his3 leu2 met15Δ0 ura3 trp1-1 can1-100 PDR5-GFP::HIS3 rtg2Δ::KanMX4 yap1Δ::KanMX4 | Pdr5-GFP Δrtg2, Δyap1 |
Yor1-GFP | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 YOR1-GFP::HIS3 | Huh et al.26 |
