Figure 5
From: Injectable cartilaginous template transformed BMSCs into vascularized bone
Cell bricks guided persistent deposition and production of bony collagen. Examination of the osteogenesis of grafts in vivo at 4 weeks (A–H) and 12 weeks (I–P). (A,C,D,I,K,L) The PDCM-CB-P constructs exhibited ossification through 12 weeks, as confirmed by the high expression of bony collagen, such as COL-I and COL-X, and the lower expressed COL-II in immunostaining. (B,J) Sirius red staining revealed that COL-II was replaced by COL-I and COL-X. (E–H,M–P) The PDCM-P constructs exhibited slower ossification than the PDCM-CB-P groups, bar = 200 μm, magnification = 10×. PDCM, particulate decellularised cartilage matrix; CB, cell brick; P, platelet-rich plasma; COL, collagen.
