Figure 1
From: Cytosolic Genomic DNA functions as a Natural Antisense
The cytosolic fraction does not contain nuclear components. (A) The experimental scheme of fractionation. (B) Fractions from three mouse cell lines were used for cgDNA PCR amplification with mouse Gapdh intron specific forward and reverse primers. (C) Immunoblotting of nuclear and cytosolic fractions. (D) Sequences of 721 in nucleus and cytosols from Hepa1-6, Neuro2A, and C2C12 cells were amplified by PCR. (E) Semi-quantitative analysis of cgDNA. Images were analyzed by using ImageJ software. The upper panel represents a dot plot. The lower panel represents a normalized bar graph. (F) Cytosolic genomic transposon DNA was amplified by PCR.
