Figure 2
From: Cytosolic Genomic DNA functions as a Natural Antisense
Gene mapping of 721 sequence on chromosome 19. (A) 721 sequence located on the upstream of Cytochrome c oxidase subunit VIIIa (Cox8a) and the downstream of N(alpha)-acetyltransferase 40, NatD catalytic subunit (Naa40). (B) The sense strand of the 721 sequence mapped to both the sense and antisense strands of Naa40. (C) The experimental scheme of RT-qPCR. (D–F) Ctrl or 721 dsDNA 4 nM were transfected to C2C12 mouse muscle cells. RNA was extracted 24 h post-transfection. Quantitative analysis of the liDNA effect for (D) 721, (E) Cox8a and (F) Naa40 (p < 0.05; n = 3; mean ± SD).
