Figure 3

Inhibition of Naa40 reduces cell survival. (A) Schematic representation of experiments with shRNA cell. (B and C) Protein expressions of RNase H1, MUS81, and TREX1 in knocked down cells. (B) C2C12 cells were used to generate subcell lines stably expressing shRNA against luciferase, RNase H1, MUS81, and TREX1. Immunoblots performed with extracts from shRNA knocked down C2C12 cells. (C) Immunoblots performed with extracts from transient shRNA knocked down Hepa1-6 cells. (D) For proliferation studies, cells were plated and incubated overnight, and baseline counts were taken the following day (Day 0). Cell populations at Day 3 were normalized to those at Day 0. Cell counts for shluc versus shRNaseH1, shMUS81 and shTREX1 expressing C2C12 cells (**p < 0.01 by ANOVA/Bonferroni test; n = 3; mean ± SD). (E) Quantitative analysis (qPCR) of abundance of 721 in the cytosolic fractions (*p < 0.01 by ANOVA/Bonferroni test; n = 4; mean ± SD). (F) Quantitative analysis (RT-qPCR) of the 721 expression in knocked down cells (*p < 0.05 and **p < 0.01 by ANOVA/Bonferroni test; n = 4; mean ± SD).